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Correlation between solvent production and level of solventogenic enzymes in Clostridium acetobutylicum
- Ballongue, J., Janati-Idrissi, R., Petitdemange, H., Gay, R.
- Journal of applied microbiology 1989 v.67 no.6 pp. 611-617
- Clostridium acetobutylicum, acetates, acids, bacteria, butanol, butyrates, enzymes, glucose, pH, solvents
- Clostridium acetobutylicum is an anaerobic bacterium which converts glucose into acids at a neutral pH and into solvents at a pH lower than 5·0. This paper shows the evolution of the levels of enzymes responsible for solvent formation: acetoacetate decarboxylase, acetoacetyl CoA acetate (butyrate) coenzyme A transferase and butanol dehydrogenase. The study was carried out at pH 6·8 under the conditions for acid formation, and at pH 4·8 the condition for solvent formation. These enzymes are present at both pH levels, at pH 6·8 this presence is not accompanied by any correlative solvent production.