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Cytochrome cbb₃ of Thioalkalivibrio is a Na⁺-pumping cytochrome oxidase

Author:
Muntyan, Maria S., Cherepanov, Dmitry A., Malinen, Anssi M., Bloch, Dmitry A., Sorokin, Dimitry Y., Severina, Inna I., Ivashina, Tatiana V., Lahti, Reijo, Muyzer, Gerard, Skulachev, Vladimir P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2015 v.112 no.25 pp. 7695-7700
ISSN:
0027-8424
Subject:
Thioalkalivibrio, cytochrome c, cytochrome-c oxidase, electron transport chain, energy conversion, oxygen, proton-motive force
Abstract:
Cytochrome c oxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases ( cbb ₃ cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), a cbb ₃ cytochrome from the extremely alkaliphilic bacterium Thioalkalivibrio versutus . This finding offers clues to the previously unknown structure of the ion-pumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.
Agid:
2913174