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Antimicrobial activity and the major components of the essential oil of Plectranthus cylindraceus

Author:
Marwah, R.G., Fatope, M.O., Deadman, M.L., Ochei, J.E., Al-Saidi, S.H.
Source:
Journal of applied microbiology 2007 v.103 no.4 pp. 1220-1226
ISSN:
1364-5072
Subject:
Alternaria alternata, Bacillus subtilis, Bipolaris, Candida albicans, Curvularia lunata, Fusarium oxysporum, Klebsiella pneumoniae, Microsporum canis, Microsporum gypseum, Plectranthus, Staphylococcus aureus, Stemphylium solani, Trichophyton rubrum, aerial parts, animal pathogenic bacteria, anti-infective properties, carvacrol, disinfectants, essential oils, gas chromatography-mass spectrometry, growth retardation, minimum inhibitory concentration, nuclear magnetic resonance spectroscopy, odors, oils, plant extracts, stable isotopes, yeasts, Oman
Abstract:
The traditional uses of the aerial parts of fresh Plectranthus cylindraceus by the Dhofaris in Oman were investigated on the basis of antimicrobial properties and composition of its herb oil. The minimal inhibitory concentration (MIC) values of the herb oil of P. cylindraceus against human pathogenic bacteria and yeast were assessed using the broth microdilution method, and the percentage growth inhibition of fungi was determined according to the poisoned food technique. The oil exhibited good activity against Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis and Candida albicans with MIC values in the range of 7·8-62·5 μg ml⁻¹ and also inhibited the growth of Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Alternaria alternata, Bipolaris sp., Curvularia lunata, Fusarium oxysporum and Stemphylium solani for about a week at 250 μg ml⁻¹. The two most abundant components of the oil were identified as carvacrol (46·8%) and α-terpinolene (18·2%) based on gas chromatography-mass spectra (GC-MS) and ¹³C nuclear magnetic resonance (NMR) analyses. The oil has a pleasant odour and showed broad spectrum antimicrobial activity. Such bioactivity could be attributed in part to carvacrol. This study has demonstrated the broad spectrum antimicrobial activity of the herb oil of P. cylindraceus as well as the organic composition of this plant extract. This study provides scientific insight into the ancient practice of utilizing P. cylindraceus as a fragrant disinfectant.
Agid:
2926753