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Rapid estimation of spoilage bacterial load in aerobically stored meat by a quantitative polymerase chain reaction
- Venkitanarayanan, K.S., Faustman, C., Crivello, J.F., Khan, M.I., Hoagland, T.A., Berry, B.W.
- Journal of applied microbiology 1997 v.82 no.3 pp. 359-364
- bacteria, correlation, electrochemiluminescence, meat, microbial load, nucleotide sequences, plate count, quantitative polymerase chain reaction, ribosomal DNA, spoilage, spoilage bacteria
- We report a quantitative PCR which utilizes primers from a conserved 23S rDNA sequence identified in nine different spoilage bacteria commonly present in meat. The PCR detected the spoilage bacteria by amplifying a specific 207 bp sequence from their chromosomal DNA. Quantification of PCR product by electrochemiluminescence revealed that the concentration of the amplified product was dependent on cycle number and the initial number of bacteria present in the sample. Statistical analysis of the results indicated a correlation coefficient of 0(.)94 (P < 0(.)001) between aerobic plate count and QPCR luminosity units.