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High affinity iron-uptake systems in Vibrio damsela: role in acquisition of iron from transferrin

Fouz, B., Biosca, E.G., Amaro, C.
Journal of applied microbiology 1997 v.82 no.2 pp. 157-167
Arthrobacter, Photobacterium damselae subsp. damselae, avirulent strains, chelating agents, deferoxamine, fish, hosts, iron, mammals, marine ecosystems, mutants, outer membrane proteins, pathogens, siderophores, transferrin, virulence
In this work, the high affinity iron-acquisition systems displayed by virulent and avirulent strains of Vibrio damsela have been investigated. This species is an autochthonous member of marine ecosystems that can behave as an opportunistic pathogen for fish and mammals. All strains tested (i) were able to grow under the restricted conditions imposed by the iron chelators transferrin (Tf) and EDDHA, (ii) secreted siderophores of hydroxamic type, other than aerobactin and desferal, that were able to stimulate the growth of the auxotroph mutant Arthrobacter favescens JG9, and (iii) expressed common iron-regulated outer membrane proteins (IROMPs). No change in LPS patterns was observed in response to iron restriction. Results from the assays with transferrin suggest that these siderophores could be utilized to sequester iron from Tf, a protein for which no surface receptor was detected in any strain. In summary, the overall data demonstrate that V. damsela expresses siderophore-mediated iron-uptake systems. These systems are probably involved in the survival of the species in the different environments that it can colonize, i.e. water and several vertebrate hosts.