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Volatile constituents and biological activities of Pycnostachys abyssinica and Pycnostachys eminii extracts

Author:
Hussien, Jemal, Hymete, Ariaya, Rohloff, Jens
Source:
Pharmaceutical biology 2010 v.48 no.12 pp. 1384-1391
ISSN:
1388-0209
Subject:
Lamiaceae, acetates, alkaloids, anthelmintics, anti-infective properties, beta-caryophyllene, bioassays, carotenoids, chloroform, diterpenoids, dysentery, earthworms, essential oils, eyes, flavonoids, gas chromatography-mass spectrometry, germacrene, hydrodistillation, leaves, medicine, methanol, methyl chavicol, petroleum, phytosterols, plant organs, polyphenols, saponins, screening, solvents, Ethiopia
Abstract:
Context: Pycnostachys abyssinica Fresen and Pycnostachys eminii Gürke (Lamiaceae) are used in traditional Ethiopian medicine against eye and skin infections, “Mitch disease”, and dysentery. Objective: Our study was aimed at characterizing essential oil (EO), phytochemical groups, and antimicrobial and anthelmintic activity of extracts to underscore the species’ indigenous medicinal use. Materials and methods: Plant organs of Pycnostachys species were subjected to hydrodistillation, and essential oils (EO) analyzed by GC-MS. Phytochemical compounds, antimicrobial (diffusion assay) and anthelmintic activity (bioassay) of gradient solvent extracts of different polarity were studied. Results: In the stem and root EO of P. abyssinica, 25 (99%) and 30 (99.79%) compounds were detected respectively, with estragole (70.4%) (stem) and exo-fenchyl acetate (30.6%) (root) as the most abundant compounds. In leaf, stem and root EO of P. eminii, 30 (90.66%), 27 (90.59%) and 27 (99.96%) compounds were detected, respectively, with high levels of β-caryophyllene (from 18.08% to 28.85%) and germacrene D (from 15.1% to 22.06%). Alkaloids, saponins, phytosterols, flavonoids, polyphenols, diterpenoids and carotenoids were detected in Pycnostachys. Petroleum ether, chloroform and methanol extracts showed distinct antimicrobial effects with generally higher potential activity of lipophilic and semi-lipophilic fractions. Leaf and root methanol extracts of both species showed lethal activity against earthworms. Discussion: Identified EO constituents and phytochemical groups underscore the observed antifungal, antibacterial and anthelmintic activity of Pycnostachys gradient solvent extracts. Conclusion: EO analysis, phytochemical screening, and antimicrobial and anthelmintic assays indicate the biological potential of Pycnostachys species from Ethiopia, and emphasize their pharmacological and indigenous applications.
Agid:
293748