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Anatomy of Accessory Salivary Glands of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) and Correlations to Begomovirus Transmission

Author:
Cicero, Joseph M., Brown, Judith K.
Source:
Annals of the Entomological Society of America 2011 v.104 no.2 pp. 280-286
ISSN:
0013-8746
Subject:
Begomovirus, Bemisia tabaci, Hemiptera, abdomen, direct contact, light microscopy, midgut, salivary glands, salivation, thorax, toluidine blue, transmission electron microscopy, virus transmission
Abstract:
Visualization of dissected accessory salivary glands (ASGs) of the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) by light microscopy (LM) revealed three distinctive toluidine blue O stain profiles. Considered morphotypes, the three profiles are hypothesized to represent stages of a salivation cycle, wherein contents are cyclically depleted and subsequently regenerated as needed for feeding. When whiteflies were repeatedly interrupted during their initial feeding behaviors, and then ASGs were dissected, a fourth stain profile was revealed. These observations are therefore relevant to the different mechanisms involved in whitefly-mediated virus transmission to plants. Stain techniques involved in transmission electron microscopy of extirpated and nonextirpated ASGs reveal entirely different profiles that cannot yet be correlated to LM findings. The midgut of B. tabaci is capable of transposing its location from the abdomen to the thorax and can come into direct contact with the ASGs. This finding opens new lines of thought in the potential for interaction between the two, such as purging of excess water and waste, and virus transmission.
Agid:
294036