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Termite repellent sesquiterpenoids from Callitris glaucophylla heartwood

Author:
Watanabe, Yasutaka, Mihara, Rie, Mitsunaga, Tohru, Yoshimura, Tsuyoshi
Source:
Journal of wood science 2005 v.51 no.5 pp. 514-519
ISSN:
1435-0211
Subject:
termite control, Coptotermes formosanus, Callitris glaucophylla, heartwood, sesquiterpenoids
Abstract:
Fractions of methanol and ethanol extracts from the heartwood of white cypress pine (Callitris glaucophylla Thompson et Johnson) were investigated for their repellent activity against subterranean termite Coptotermes formosanus Shiraki worker using a two-choice semicircular filter paper test at 0.5% (w/w) concentration. Fraction CY-E2 composed of (−)-citronellic acid, guaiol, α-, β-, and γ-eudesmol isomers as well as an unknown compound, showed the highest statistically significant repellency (97.8% ± 2.2 SEM) of all fractions tested. Bioactivity-guided fractionations using high-performance liquid chromatography led to the isolation of two, oxygenated eudesmane-type sesquiterpenes with α-methylene moieties, both termite-repellent compounds. These compounds were subsequently identified as ilicic acid methyl ester (IAME) and costic acid by means of spectroscopic analyses, electron impact mass spectrometry, and nuclear magnetic resonance spectroscopy. We report the isolation of both IAME and costic acid from C. glaucophylla heartwood for the first time.
Agid:
2984391