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Probing Allostery Through DNA

Author:
Kim, Sangjin, Broströmer, Erik, Xing, Dong, Jin, Jianshi, Chong, Shasha, Ge, Hao, Wang, Siyuan, Gu, Chan, Yang, Lijiang, Gao, Yi Qin, Su, Xiao-dong, Sun, Yujie, Xie, X. Sunney
Source:
Science 2013 v.339 no.6121 pp. 816-819
ISSN:
0036-8075
Subject:
DNA, DNA-directed RNA polymerase, bacteria, binding capacity, gene expression, periodicity, repressor proteins
Abstract:
Allostery Across DNA Proteins, such as transcription factors and RNA polymerase, bind close to each other on DNA and their function is coordinated. Kim et al. (p. 816; see the Perspective by Crothers) report single-molecule experiments that show that the DNA binding affinity of a protein is significantly altered by a second protein bound nearby. The effect oscillates between stabilizing and destabilizing the binding with a periodicity equal to the helical pitch of DNA. Allosteric coupling between a transcriptional repressor and RNA polymerase modulated gene expression in living bacteria.
Agid:
3008748