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A Genetic Variation Analysis of V. vinifera cv. Pinot noir

Forneck, Astrid, Konradi, J., Blaich, R.
Acta horticulturae 2003 no.603 pp. 167-170
Vitis vinifera, asexual reproduction, breeding, clones, cultivars, genetic variation, grapes, phenotype, vegetative propagation
This project aims to analyze clonality in the long lived heterozygous perennial Vitis vinifera L. (Vitaceae). Vegetatively propagated grapevines have been subject to clonal selection resulting in a large number of clones that diverge in yield, quality and morphologically distinct phenotypes. Economic clonal selection in grapes has been performed since the early 20th century and led to hundreds of grapevine clones. There is great interest in ways to individually fingerprint these clones, which cannot be distinguished through morphological traits. In order to identify grapevine clones with molecular marker systems, we first investigate the degree of genetic diversity that exists within such clones, that have undergone solely clonal reproduction. Then, genetic variation between grapevine clones is analysed and the polymorphisms found are further characterized to understand the nature and frequency of this genetic change that occurred during long-term vegetative propagation. The identification and preservation of clonal diversity is important to sustain genetic diversity in a monocultural and intensively cultivated crop. Multiple clones of a cultivar are important to meet the differing needs of viticulturists and offer chances for grape breeding combined with research on molecular genetic levels. Here we report a preview of our work on the use of AFLP-PCR markers in the study of genetic diversity within grapevine clones of V. vinifera cv. Pinot noir.