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Genetic analysis of phylloxera root resistance in cultivar 'Borner'

Hausmann, L., Eibach, R., Zyprian, E., Topfer, R.
Acta horticulturae 2011 no.904 pp. 47-52
Daktulosphaira vitifoliae, Vitis cinerea, Vitis riparia, cultivars, leaf galls, loci, marker-assisted selection, microsatellite repeats, molecular cloning, progeny, roots, rootstocks
Most rootstock cultivars currently used show root nodosity symptoms after phylloxera infection. However, rootstock cultivar ‘Börner’, an interspecific cross of Vitis riparia ‘183G’ and Vitis cinerea ‘Arnold’, is regarded as phylloxera-resistant as it shows neither tuberosity nor nodosity symptoms. A classical map-based cloning approach was used to identify the genetic determinants of phylloxera root resistance in ‘Börner’. The roots of the progeny of a cross of Gf.V.3125 (‘Schiava grossa’ × ‘Riesling’) × ‘Börner’ were artificially infested with phylloxera leaf galls (containing phylloxera) and phenotyped qualitatively and quantitatively for nodosity formation. After establishing a genetic map of the parents a single significant locus named Rdv1 (Resistance Daktulosphaira vitifoliae) was localized on chromosome 13 in the map of ‘Börner’ (Zhang et al., 2009). Simple sequence repeat (SSR) marker comparison between ‘Börner’ and the parents V. riparia ‘183G’ and V. cinerea ‘Arnold’ confirmed the latter as the origin of Rdv1. Fine mapping of the Rdv1 locus was started with synteny-derived STS (sequence-tagged site) markers deduced from the PN40024 reference sequence. Markers, tightly linked to the resistance locus, were obtained and found to be useful for marker-assisted selection in breeding programs.