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Efficacy of Bacteriophage Therapy on Horizontal Transmission of Salmonella Gallinarum on Commercial Layer Chickens

Lim, Tae-Hyun, Lee, Dong-Hun, Lee, Yu-Na, Park, Je-Keun, Youn, Ha-Na, Kim, Myung-Seob, Lee, Hyun-Jeong, Yang, Si-Yong, Cho, Young-Wook, Lee, Joong-Bok, Park, Seung-Yong, Choi, In-Soo, Song, Chang-Seon
Avian diseases 2011 v.55 no.3 pp. 435-438
Salmonella Gallinarum, antibiotics, bacteriophages, bird diseases, cecum, disease transmission, feed additives, laying hens, liver, mortality, sewage effluent, spleen, therapeutics
A Salmonella Gallinarum (SG)-specific bacteriophage isolated from sewage effluent was used to prevent horizontal transmission of SG in commercial layer chickens. Six-week-old chickens, each challenged with 5 × 108 colony-forming units of SG, cohabited with contact chickens treated with 106 plaque-forming units/kg of bacteriophage, prepared in feed additives, for 7 days before, and 21 days after challenge with SG. Mortality was observed for 3 wk after challenge and SG was periodically re-isolated from the liver, spleen, and cecum of chickens. SG re-isolation from organs was decreased and a significant (P < 0.05) reduction in mortality was observed in contact chickens treated with the bacteriophage, as compared to untreated contact chickens, indicating that bacteriophage administration in feed additives significantly prevented the horizontal transmission of SG. These results provide important insights into prevention and control strategies against SG infection and suggest that the use of bacteriophages may be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of SG infection in poultry.