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Low EOF rate measurement based on constant effective mobility in microchip CE

Author:
Wang, Wei, Zhao, Liang, Zhou, Fang, Zhang, Jian-Rong, Zhu, Jun-Jie, Chen, Hong-Yuan
Source:
Electrophoresis 2007 v.28 no.16 pp. 2893-2896
ISSN:
0173-0835
Subject:
buffers, capillary electrophoresis, equations, semiconductors
Abstract:
A new method for quickly determining low EOF rates (μEOF) in microchip CE is described. The measurement is based on the notion that the effective mobility (μeff) of an analyte is a constant in a certain BGE. The μeff of an analyte is determined in a reference fast-electroosmosis microchip, and the apparent mobility (μapp) of the analyte can be determined in the microchip with unknown low electroosmosis, and then μEOF in the low-electroosmosis microchip can be calculated according to the equation μEOF = μapp - μeff. By an indirect method or other conventional methods, μeff can be easily measured in the reference microchip. The proposed method is particularly useful for low-electroosmosis measurements in wall-modified microchannels.
Agid:
3039992