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In-channel atom-transfer radical polymerization of thermoset polyester microfluidic devices for bioanalytical applications
- Pan, Tao, Fiorini, Gina S., Chiu, Daniel T., Woolley, Adam T.
- Electrophoresis 2007 v.28 no.16 pp. 2904-2911
- X-ray photoelectron spectroscopy, adsorption, amino acids, capillary electrophoresis, contact angle, derivatization, ethylene glycol, hydrophilicity, phosphorylation, polyesters, polymerization
- A new technique for polymer microchannel surface modification, called in-channel atom-transfer radical polymerization, has been developed and applied in the surface derivatization of thermoset polyester (TPE) microdevices with poly(ethylene glycol) (PEG). X-ray photoelectron spectroscopy, electroosmotic flow (EOF), and contact angle measurements indicate that PEG has been grafted on the TPE surface. Moreover, PEG-modified microchannels have much lower and more pH-stable EOF, more hydrophilic surfaces and reduced nonspecific protein adsorption. Capillary electrophoresis separation of amino acid and peptide mixtures in these PEG-modified TPE microchips had good reproducibility. Phosducin-like protein and phosphorylated phosducin-like protein were also separated to measure the phosphorylation efficiency. Our results indicate that PEG-grafted TPE microchips have broad potential application in biomolecular analysis.