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IPG with electrodic plateaus (and other unusual procedures for 2-DE)

Author:
Gianazza, Elisabetta, Rocco, Alessandro Guerini, Marchetto, Andrea, Vergani, Lodovica
Source:
Electrophoresis 2007 v.28 no.16 pp. 2953-2956
ISSN:
0173-0835
Subject:
electrophoresis, polymerization, rehydration, trays
Abstract:
We describe some simple changes to the geometry of the IPG strips that make them suitable to the loading of very large sample volumes and of high-salt solutions. Of special relevance is the possibility of using strips with immobilized plateau(s) to either side of the gradient, or to both, also in connection with in-gel rehydration protocols and focusing in stock trays. The only requirement to achieve this is to leave the all-ready-made attitude and go back to custom polymerization of the IPGs in one's laboratory.
Agid:
3040001