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The use of molecular tools for a better management of the French Daucus genetic resources network

Le Clerc, V., Briard, M.
Acta horticulturae 2003 no.598 pp. 193-197
Alternaria dauci, Daucus carota, botanical gardens, business enterprises, environmental factors, experts, gene banks, genes, genetic drift, genetic markers, genetic relationships, genetic variation, genotype, germplasm conservation, researchers, France
In numerous countries genetic resources are conserved in centralised gene banks, whereas in France specialised networks do their management. This original organisation allows sharing costs and working between each partner for maintenance and evaluation. Moreover, experts on the considered species manage each network. The French Daucus network, focusing on Daucus carota L., was created a few years ago. Its goal was to merge the independent Daucus germplasm collections belonging to different partners: several French seed companies, some botanical gardens and our institute. In order to be useful for researchers and breeders or other potential users, this national collection needs to be characterised, well conserved to preserve the genetic integrity of each accession, and evaluated. Traditionally, morphological characters are used to characterise the genotypes, but they can be affected by environmental factors. To obtain a more reliable estimation of the diversity and genetic relationships, we developed molecular markers. They were shown to be useful not only for the characterisation of the accessions but also for genetic drift control during regeneration. To valorise this collection an evaluation of resistance to Alternaria dauci was also engaged. The efficiency of molecular markers linked to resistance genes is under evaluation.