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Worldwide Core Collection of Olive Cultivars Based on Simple Sequence Repeat and Morphological Markers

Author:
Diez, Concepcion M., Imperato, Annalisa, Rallo, Luis, Barranco, Diego, Trujillo, Isabel
Source:
Crop science 2012 v.52 no.1 pp. 211-221
ISSN:
1435-0653
Subject:
Olea europaea, algorithms, alleles, computer software, cultivars, data collection, genotype, germplasm, germplasm conservation, microsatellite repeats, olives, Spain
Abstract:
Core collections reflecting the diversity of large germplasm collections are valuable tools to conserve and evaluate the genetic resources more effectively. Three strategies using two different search algorithms were trained to develop the most representative nested core collection of the World Olive Germplasm Bank (WOGB) of Cordoba (Spain), the largest and best characterized collection of olive (Olea europaea L.) cultivars worldwide. The original dataset was composed of 378 olive accessions from 21 different countries characterized by 14 microsatellite markers (simple sequence repeats [SSRs]). Each nested core collection included five subsets representing different percentages of the base collection (5, 10, 15, 20, and 30%). The strategy maximizing the allele coverage implemented in the software Core Hunter was chosen as the best. The five subsets developed by this method captured the maximum number of alleles showing fewer significant differences in their frequencies with respect to the whole collection. Additionally, a balanced geographical composition was observed in the nested subsets in spite of the strong overrepresentation of western Mediterranean accessions present in the WOGB. The results of this study are aimed at improving both the conservation and management of olive genetic resources and the study of differential genotype × environment interactions by testing a minimum number of accessions.
Agid:
307480