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Development and characterization of microsatellite markers for parentage analyses of the coral reef damselfish (Pomacentrus amboinensis: Pomacentridae)

Author:
Munkres, Katherine P., Bay, Line K., Jerry, Dean R., McCormick, Mark I., Van Herwerden, Lynne
Source:
Conservation genetics 2007 v.8 no.4 pp. 987-990
ISSN:
1566-0621
Subject:
Pomacentrus, Stegastes, alleles, coral reefs, genetic markers, genotyping, heterozygosity, loci, microsatellite repeats, parentage, Great Barrier Reef
Abstract:
Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles per locus ranging from 6-23 and observed heterozygosity between 0.42-0.92. In addition, the cross-species testing of six primers developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (>=14 alleles) in at least four of the six loci and a high proportion of tetranucleotide repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual relatedness.
Agid:
3089929