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Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae

Author:
Liao, Xiaolin, Wang, Dan, Yu, Xiaomu, Li, Weitao, Cheng, Lei, Wang, Jianwei, Tong, Jingou
Source:
Conservation genetics 2007 v.8 no.4 pp. 1003-1007
ISSN:
1566-0621
Subject:
Gobiocypris rarus, alleles, genetic variation, genomic libraries, indigenous species, loci, microsatellite repeats, minnows, population structure, Yangtze River
Abstract:
Rare minnow (Gobiocypris rarus) is an endangered small fish endemic to upper reach of the Yangtze River. From a (GT)n enriched genomic library, 32 microsatellites were isolated and characterized. Nineteen of these loci were polymorphic in a test population with alleles ranging from 2-7, and observed and expected heterozygosities from zero to 0.8438, and 0.2679 to 0.8264, respectively. In the cross-species amplifications, 13 out of 19 polymorphic loci were found to be also polymorphic in at least one of the 7 closely related species of the subfamily Gobioninae. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to evaluate the fine-scale population structure in rare minnow and its closely related species for the conservation purpose.
Agid:
3089980