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Isolation and characterization of microsatellite loci in Melicope quadrilocularis (Rutaceae), an endemic plant species of the Bonin Islands, Japan, and cross-species amplification in closely related taxa

Author:
Katoh, Saeko, Kaneko, Shingo, Isagi, Yuji, Murakami, Noriaki, Kato, Hidetoshi
Source:
Conservation genetics 2007 v.8 no.6 pp. 1487-1490
ISSN:
1566-0621
Subject:
Melicope, alleles, heterozygosity, indigenous species, islands, loci, microsatellite repeats, trees, Japan
Abstract:
Twelve microsatellite loci were isolated and characterized for Melicope quadrilocularis, an insular endemic tree species of the Bonin Islands. The observed number of alleles at each locus ranged from 1 to 18. The range of expected heterozygosity was 0.0000-0.9445. The inter-specific applicability of these loci was evaluated by analyzing two other endemic species and one endemic variety of Melicope that are also distributed on the Bonin Islands. All primer pairs for the 12 loci tested successfully amplified the loci in all taxa, except for primers for four loci in M. nishimurae.
Agid:
3090032