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A Biochemical Comparison of Proteases from Pathogenic Naegleria fowleri and Non-Pathogenic Naegleria gruberi

Author:
SERRANO-LUNA, JESÚS, CERVANTES-SANDOVAL, ISAAC, TSUTSUMI, VICTOR, SHIBAYAMA, MINEKO
Source:
Journal of eukaryotic microbiology 2007 v.54 no.5 pp. 411-417
ISSN:
1066-5234
Subject:
Naegleria fowleri, central nervous system, cysteine proteinases, enzyme activity, humans, meningoencephalitis, molecular weight, nutrients, pH, parasites, pathogenesis, polyacrylamide gel electrophoresis, proteolysis, serine proteinases, temperature, trophozoites
Abstract:
Naegleria fowleri is the etiologic agent of primary amoebic meningoencephalitis (PAM). Proteases have been suggested to be involved in tissue invasion and destruction during infection. We analyzed and compared the complete protease profiles of total crude extract and conditioned medium of both pathogenic N. fowleri and non-pathogenic Naegleria gruberi trophozoites. Using SDS-PAGE, we found differences in the number and molecular weight of proteolytic bands between the two strains. The proteases showed optimal activity at pH 7.0 and 35 °C for both strains. Inhibition assays showed that the main proteolytic activity in both strains is due to cysteine proteases although serine proteases were also detected. Both N. fowleri and N. gruberi have a variety of different protease activities at different pH levels and temperatures. These proteases may allow the amoebae to acquire nutrients from different sources, including those from the host. Although, the role of the amoebic proteases in the pathogenesis of PAM is not clearly defined, it seems that proteases and other molecules of the parasite as well as those from the host, could be participating in the damage to the human central nervous system.
Agid:
3102452