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Aconitic acid determination by gas-liquid chromatography

Author:
Mehltretter, C.L., Otten, J.G.
Source:
International sugar journal 1971 v.73 no.872 pp. 235
ISSN:
0020-8841
Subject:
sugars, aconitic acid, chemical analysis, gas chromatography, mass spectrometry, aqueous solutions, molasses, syrups, grain sorghum
Abstract:
Quantitative isolation of aconitic acid in molasses and sugar syrups as insoluble lead aconitate is carried out using a modification of the method of Gupta and Chetal [Int. Sug. J. (1968) 70: 107-110]. 12.5 g molasses are dissolved in 50 cm3 distilled water, made up to 100 cm3 with distilled water, a 10 cm3 aliquot is diluted with 10 cm3 distilled water, adjusted to pH 10-11 by addition of 0.2 cm3 of 10% NaOH solution, treated with 5 cm3 of 10% lead acetate trihydrate solution, shaken for 5 min, centrifuged at 1500 rev/min for 5 min, and the precipitate washed with distilled water and dried. GLC analyses gave the following results % (3 samples and average respectively): Egyptian final molasses, 0.50, 0.58, 0.53, 0.54; Louisiana A-molasses, 1.94, 1.90, 1.96, 1.93; sorghum syrup, 1.01, 1.00, 0.95, 0.99.
Agid:
31072
Handle:
10113/31072