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Establishment of four pairs of canine mammary tumour cell lines derived from primary and metastatic origin and their E-cadherin expression
- Uyama, R., Nakagawa, T., Hong, S.-H., Mochizuki, M., Nishimura, R., Sasaki, N.
- Veterinary and comparative oncology 2006 v.4 no.2 pp. 104-113
- breast neoplasms, cadherins, cultured cells, dogs, fetal bovine serum, flow cytometry, mammary neoplasms (animal), metastasis, neoplasm cells
- Four new pairs of canine mammary carcinoma cell lines derived from both primary and metastatic lesions were established. The cells were cultured in RPMI-1640 with 10% fetal bovine serum and they showed stable growth for more than 120 passages. Using these cell lines, the expression of E-cadherin was measured by flow cytometry and the function of E-cadherin was evaluated by cell aggregation assay and results from the primary and metastatic lesions were compared statistically. E-cadherin was strongly expressed in all of the cell lines, without a notable difference between cells of primary and metastatic origin. In the cell aggregation assay, the function of E-cadherin was significantly weaker in the cells of primary origin (p < 0.05), as compared with cells of metastatic origin. The present results suggest that a reduction in E-cadherin function may be implicated in the invasive and metastatic potential of canine mammary tumour cells; however, further study will be needed to clarify E-cadherin function in the context of the metastasis of canine mammary carcinoma.