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Soybean trypsin inhibitor: a reference protein for gel electrophoretic studies of soybean proteins
- Eldridge, A.C., Kalbrener, J.E., Wolf, W.J.
- Cereal chemistry 1970 v.47 no.2 pp. 167
- soybeans, trypsin inhibitors, protein composition, gel electrophoresis, plant proteins
- Earlier studies showed commercial samples of crystalline soybean trypsin inhibitor (SBTI) to be heterogeneous. An automated diethylaminoethyl (DEAE) cellulose chromatographic system was used to purify several commercial samples of SBTI. The SBTIs purified by column chromatography were analysed by 7 different gel electrophoretic procedures. The protein migrated as a characteristic, fast-moving band in most gel systems studied. SBTI can be used as a reference protein to calculate the relative mobilities of other protein bands in electrophoretic studies of soybean proteins.