Main content area

Efficient production of doubled haploid melon plants by modified colchicine treatment of parthenogenetic haploids

Yashiro, K., Hosoya, K., Kuzuya, M., Tomita, K., Ezura, H.
Acta horticulturae 2002 no.588 pp. 335-338
Cucumis melo, X-radiation, breeding methods, colchicine, cultivars, fruits, greenhouses, haploidy, ovules, parthenogenesis, plant breeding, planting, pollen, seedlings, shoots, soaking
F1 hybrid cultivars have been widely used for commercial melon (Cucumis melo) production. A pure line is essential for F1 seed production. However, it takes more than seven years to produce such pure lines using conventional plant breeding techniques. Therefore, a method for producing parthenogenetic haploid plants has been developed. In this study, we report a method for efficiently producing doubled haploid (DH) plants by colchicine treatment of haploid plants. Fruits of five F1 cultivars pollinated with soft X-ray (65 KR) irradiated pollen were harvested, and the ovules were cultured in vitro. Fifty-one haploid seedlings were obtained from about 10,000 ovules from twenty fruits in each cultivar. For five cultivars, the rates of haploid production ranged from 0.03% to 0.17%. Twenty seedlings became vitrified or died while growing in vitro. In total, 31 haploid plants were obtained and planted in a greenhouse for doubling. The haploid lines were treated by soaking the tips of many lateral shoots in a 0.1% colchicine solution that contained a small amount of detergent. Of 31 lines, 21 were doubled following colchicine treatment, 68% of the treated plants.