Jump to Main Content
Cloning and characterization of a melon E8-like gene
- Fujimori, A., Pariasca, J.A.T., Iida, M., Uchiyama, S., Ozawa, T., Hirabayashi, T., Nakagawa, H., Sato, T.
- Acta horticulturae 2002 no.588 pp. 363-367
- tomatoes, messenger RNA, DNA, reverse transcriptase polymerase chain reaction, introns, amino acid sequences, amino acids, mesocarp, ripening, exons, apricots
- A genomic DNA encoding an E8-like gene (CmE8, accession number AB071820) was isolated from melon and its nucleotide sequence was determined. The CmE8 consisted of three exons and two introns. The number and size of the exons and the location of introns were identical to those in tomato E8 gene. The deduced amino acid sequence of CmE8 consisted of 367 amino acids, and it was similar to those of 2-oxoglutarate-dependent dioxygenase (2-ODD) homologues from apricot, tomato (E8 and E8-like genes) and Chataransus roseu. RT-PCR analysis indicated that mRNA levels of CmE8 increased in mesocarp of ripening melon fruit. Nucleotide sequences resembling ethylene-response elements were found in the 5’- flanking region of CmE8 gene.