Main content area

Selenium Supplementation Can Protect Cultured Rat Cardiomyocytes from Hypoxia/Reoxygenation Damage

Bordoni, Alessandra, Biagi, Pier L., Angeloni, Cristina, Leoncini, Emanuela, Muccinelli, Ivan, Hrelia, Silvana
Journal of agricultural and food chemistry 2003 v.51 no.6 pp. 1736-1740
antioxidant activity, cardiomyocytes, glutathione peroxidase, hypoxia, lipids, oxidative stress, rats, selenium, sodium selenite
The possibility of enhancing glutathione peroxidase (GPx) activity and cytosolic total antioxidant activity (TAA) in normoxia and hypoxia/reoxygenation (H/R) by the supplementation of different concentrations of sodium selenite (SS) or selenomethionine (SM) was investigated in cultured rat cardiomyocytes. To assess the entity of oxidative stress due to H/R, levels of conjugated dienes containing lipids were determined. In normoxia, GPx activity and TAA increased in parallel with the increase in SS and SM supplementation. H/R did not influence GPx activity but lowered TAA; both SS and SM supplementations were effective in increasing GPx activity, the most effective concentration being 1 μM. At this SS and SM concentration, TAA returned to a normoxic value. Conjugated diene production, increased by H/R, was reduced by SS and SM supplementation, the 1 μM concentration appearing to be the most effective one. According to these data Se supplementation represents another possibility to counteract oxidative damage in the myocardium.