Main content area

Virus incidence in four Olea europaea cultivars evaluated by mechanical inoculation and immunological assays

Félix, M.R.F., Clara, M.I.E., Leitão, F.A., Serrano, J.M. Fernandes
Acta horticulturae 2002 no.586 pp. 721-724
Arabis mosaic virus, Cherry leaf roll virus, Cucumber mosaic virus, Olea europaea, Strawberry latent ringspot virus, cultivars, enzyme-linked immunosorbent assay, groves, indicator species, orchards, plant extracts, propagation materials, surveys, trees, viruses, Portugal
A viral survey was carried out, over a period of 3 years, in adult field trees of Olea europaea L. cultivars grown in Portugal. For each cultivar groups of 10 to 15 trees per orchard, totalling 257 trees, which had been subjected to agronomical evaluation, were tested for the presence of viruses, at least twice in those 3 years. This was done by means of mechanical inoculations of plant extracts to herbaceous indicator plants, and by ELISA to detect Strawberry latent ringspot virus (SLRSV), Cherry leafroll virus (CLRV), Arabis mosaic virus (ArMV), and Cucumber mosaic virus (CMV). A total of 80 trees of cv. Santulhana in 6 orchards in the north of Portugal were tested. Results of the application of both virus tests, ELISA and mechanical transmission, showed the highest infection (100%) in one grove and the lowest (60%) in two groves. In southern Portugal, analysis of 40 trees of cv. Galega’ (4 orchards), 77 trees of ‘cv. Cordovil de Serpa (8 orchards), and 60 trees of cv. Verdeal Alentejana (6 orchards), showed the highest infection (100%) in two groves, 100% one grove and 90% in two groves, respectively. The lowest infection were 10% in two groves, 0% in three groves and 50% in two groves. Because the majority of the sampled trees did not show obvious symptoms, except for a lower vigour, latent virus infections are likely. Material for propagation purposes is now being collected only from trees that tested virus negative.