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Ion-exchange chromatography of soybean saponins
- Wolf, W.J., Thomas, B.W.
- Journal of chromatography 1971 v.56 no.2 pp. 281
- soybeans, saponins, hydrolysis, sugars, ion exchange chromatography, sapogenins
- Resolution of soybean saponins on Dowex-1 is influenced by resin particle size, flow rate, temperature and eluting agent. Separations were good when columns of -400 mesh resin were operated at 50° and eluted at slow flow rates either acetic or propionic acid. A linear gradient of 1.5 to 3 N propionic acid separated the saponins into twelve fractions, many of which consisted of two or more components when analyzed by thin-layer chromatography. Glucuronic acid, glucose, galactose rhamnose, xylose and arabinose were found in acid hydrolyzates of all fractions, but relative amounts of the neutral sugars varied in most fractions. On analysis of the aglycones, three column fractions contained only soyasapogenol A, one contained only soyasapogenol E, while four consisted of soyasapogenol B plus small amounts of soyasapogenols C and D. Another fracion yielded a previously unreported aglycone which appears related to soyasapogenol D.