PubAg

Aflatoxins: improved resolution by thin layer chromatography

Author:

Stubblefield, R.D., Shannon, G.M., Shotwell, O.L.

Source:

Journal of the Association of Official Analytical Chemists 1969 v.52 no.4 pp. 669

ISSN:

0004-5756

Subject:

thin layer chromatography, food contamination, food analysis, aflatoxins, small grains, seeds, relative humidity, temperature, aflatoxin B2, aflatoxin G1, solvents, methanol, chloroform

Abstract:

Water was added to solvent systems for thin layer chromatography of aflatoxins to achieve more reproducible results in lab. where temp. and RH vary. Resolution of the toxins also improved. Increments of water were added to solvent systems composed of acetone-chloroform (10 + 90, 12 + 88, and 15 + 85, v/v). As the water concn. was increased, separation of aflatoxins B2 and G1 improved. These 2 toxins are usually the most difficult to resolve in these solvent systems. Separations were the best with water-acetone-chloroform (1.5 + 12 + 88, v/v/v). Water added to methanol-chloroform (3 + 97, v/v) improved resolution of this solvent system but not enough for quantitative analysis, or at times, qualitative analysis. The solvent system water-methanol-ether (1 + 3 + 96, v/v/v) separated aflatoxins as well as water-acetone-chloroform (1.5 + 12 + 88, v/v/v).

Agid:

31361

Handle:

10113/31361