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Relative standard deviations in determinations of amino acids
- Kwolek, W.F., Cavins, J.F.
- Journal of the Association of Official Analytical Chemists 1971 v.54 no.6 pp. 1283
- amino acid composition, chemical analysis, cysteine, tryptophan, tyrosine, methionine, hydrolysis, seeds, reference standards, meta-analysis
- Eighteen references were selected for computation of the relative standard deviation in estimations of amino acids. Cysteine, tryptophan, tyrosine and methionine were associated with higher relative standard deviations than other amino acids. A mean relative standard deviation of about 8%, ranging from 5.9 to 14.1% depending on the amino acid, was observed between samples hydrolysed independently. The relative standard deviation between aliquots of the same hydrolysate was 2.7%. Differences in amino acids of 10 to 12% are statistically significant when 2 means, each based on 2 hydrolyses, are compared.