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A Toll-like receptor 3 agonist (polyI:C) elicits innate host responses in the spleen and lungs of chickens

Parvizi, Payvand, Mallick, Amirul Islam, Haq, Kamran, Schlegel, Benjamin, Sharif, Shayan
Canadian journal of veterinary research 2012 v.76 no.3 pp. 230-234
Toll-like receptor 3, aerosols, agonists, chickens, double-stranded RNA, genes, interferon-alpha, interferon-beta, interleukin-1, lungs, reverse transcriptase polymerase chain reaction, spleen
Double-stranded (ds) RNA interacts with host Toll-like receptor (TLR-3), leading to the induction of anti-viral host responses. The present study was designed to compare different routes of administration of a synthetic dsRNA (polyI:C) for induction of innate responses in chicken spleen and lungs. Chickens were treated with polyI:C via the aerosol, intra-air sac (i.a.s.), and intramuscular (IM) routes. Spleen and lungs were collected at 0, 2, 6, 12, and 24 h post-treatment and the expression of innate defence genes was quantified by real-time reverse transcriptase polymerase chain reaction (RT-PCR). There was an up-regulation of interferon (IFN)-β, TLR-3, and Toll/interleukin 1 receptor domain-containing adaptor protein inducing IFN-β (TRIF) at 6 h post-treatment in the spleen via IM administration of polyI:C. There was also an increase in the expression of TLR-3 and TRIF in the spleen at 2 h post-treatment via the i.a.s. route. The expression of IFN-α and TRIF was upregulated at 6 h post-treatment via the i.a.s. route in the lungs. Overall, our results indicate that administration of polyI:C can locally and systemically induce the expression of innate response genes depending on the route.