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A Toll-like receptor 3 agonist (polyI:C) elicits innate host responses in the spleen and lungs of chickens

Author:
Parvizi, Payvand, Mallick, Amirul Islam, Haq, Kamran, Schlegel, Benjamin, Sharif, Shayan
Source:
Canadian journal of veterinary research 2012 v.76 no.3 pp. 230-234
ISSN:
0830-9000
Subject:
Toll-like receptor 3, aerosols, agonists, chickens, double-stranded RNA, genes, interferon-alpha, interferon-beta, interleukin-1, lungs, reverse transcriptase polymerase chain reaction, spleen
Abstract:
Double-stranded (ds) RNA interacts with host Toll-like receptor (TLR-3), leading to the induction of anti-viral host responses. The present study was designed to compare different routes of administration of a synthetic dsRNA (polyI:C) for induction of innate responses in chicken spleen and lungs. Chickens were treated with polyI:C via the aerosol, intra-air sac (i.a.s.), and intramuscular (IM) routes. Spleen and lungs were collected at 0, 2, 6, 12, and 24 h post-treatment and the expression of innate defence genes was quantified by real-time reverse transcriptase polymerase chain reaction (RT-PCR). There was an up-regulation of interferon (IFN)-β, TLR-3, and Toll/interleukin 1 receptor domain-containing adaptor protein inducing IFN-β (TRIF) at 6 h post-treatment in the spleen via IM administration of polyI:C. There was also an increase in the expression of TLR-3 and TRIF in the spleen at 2 h post-treatment via the i.a.s. route. The expression of IFN-α and TRIF was upregulated at 6 h post-treatment via the i.a.s. route in the lungs. Overall, our results indicate that administration of polyI:C can locally and systemically induce the expression of innate response genes depending on the route.
Agid:
314841