PubAg

Main content area

Activity-Guided Isolation of Resveratrol Oligomers from a Grapevine-Shoot Extract Using Countercurrent Chromatography

Author:
Macke, Sebastian, Jerz, Gerold, Empl, Michael T., Steinberg, Pablo, Winterhalter, Peter
Source:
Journal of agricultural and food chemistry 2012 v.60 no.48 pp. 11919-11927
ISSN:
1520-5118
Subject:
bioactive properties, countercurrent chromatography, hexane, humans, resveratrol
Abstract:
An activity-guided isolation of bioactive stilbenes has been carried out with the grapevine-shoot extract Vineatrol 30. After hexane precipitation of the polymeric constituents, the stilbene mixture was separated on a preparative scale using low-speed rotary countercurrent chromatography (LSRCCC). The antiproliferative activity of the separated LSRCCC fractions was then screened in the human cancer cell line A-431, and trans-resveratrol, trans-ε-viniferin, r-2-viniferin, hopeaphenol, and miyabenol C were identified as active principles. In addition, a new class of stilbene derivatives, which exhibit a γ-lactam ring structure and exert a weak growth-inhibiting activity in A-431 cells, has been identified.
Agid:
315711