Jump to Main Content
Rate of oxygen uptake of healthy and diseased larvae of the Japanese beetle
- Weiner, B.A., Saint-Julian, G., Kwolek, W.
- Journal of invertebrate pathology 1969 v.13 no.2 pp. 250
- Popillia japonica, larvae, milky disease, Paenibacillus popilliae, infection, oxygen consumption, hemolymph, dissolved oxygen, bacterial spores, host-pathogen relationships
- The following is substantially the authors' abstract. Conventional Warburg vessels were used in Illinois to obtain oxygen-uptake data from healthy larvae of Popillia japonica Newm. and larvae infected with the milky disease caused by Bacillus popilliae. The rate of oxygen uptake, expressed as the oxygen at standard temperature and pressure taken up per hour of living larva, was 0.392 ±0.047 for healthy third-instar larvae, 0.312±0.050 for diseased third-instar larvae, 0.156±0.048 for refrigerated third-instar larvae, and 0.332 ±0.058 for early pupae. Repeated determinations on single larvae over a 6-hr, period resulted in steadily decreasing values. No significant correlation existed between oxygen uptake and the dissolved oxygen content of the haemolymph. Injury to larvae caused by insertion of a polarograph needle for dissolved oxygen determination decreased the values obtained. After one day, the rate of oxygen uptake returned to the initial level. Regression analysis failed to establish a correlation between the concentration of microorganisms in diseased third-instar larvae and the rate of oxygen uptake.