Main content area

Carbohydrate metabolism in Agrobacterium tumefaciens

Arthur, L.O., Bulla, L.A., Jr., St. Julian, G., Nakamura, L.K.
Journal of bacteriology 1973 v.116 no.1 pp. 304-313
Agrobacterium tumefaciens, carbohydrate metabolism, glucose, pentose phosphate cycle, cell respiration, oxidation, pyruvic acid, acetic acid, succinic acid, glutamic acid
The activity of pentose cycling (PC) reactions in Agrobacterium tumefaciens is much greater than that normally found in bacteria, and in this regard the organism represents a unique category. Equations specifically derived from radiorespirometric data for bacteria with high PC activity in the presence of an alternate pathway are presented. A. tumefaciens utilizes d-glucose by strictly aerobic mechanisms involving the Entner-Doudoroff (ED) and PC pathways; relative participation by the ED pathway is 55% and by the PC cycle, 44%. The 3-ketoglycose-synthesizing system in the bacterium does not affect the relative participation of these two pathways. Radiorespirometric and enzymatic analyses clearly demonstrate that the Embden-Meyerhof-Parnas pathway does not function. Studies on the oxidation of pyruvic, acetic, succinic, and glutamic acids show that terminal respiration includes both the tricarboxylic acid and glyoxylic acid cycles.