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Carbohydrate metabolism in Agrobacterium tumefaciens
- Arthur, L.O., Bulla, L.A., Jr., St. Julian, G., Nakamura, L.K.
- Journal of bacteriology 1973 v.116 no.1 pp. 304-313
- Agrobacterium tumefaciens, carbohydrate metabolism, glucose, pentose phosphate cycle, cell respiration, oxidation, pyruvic acid, acetic acid, succinic acid, glutamic acid
- The activity of pentose cycling (PC) reactions in Agrobacterium tumefaciens is much greater than that normally found in bacteria, and in this regard the organism represents a unique category. Equations specifically derived from radiorespirometric data for bacteria with high PC activity in the presence of an alternate pathway are presented. A. tumefaciens utilizes d-glucose by strictly aerobic mechanisms involving the Entner-Doudoroff (ED) and PC pathways; relative participation by the ED pathway is 55% and by the PC cycle, 44%. The 3-ketoglycose-synthesizing system in the bacterium does not affect the relative participation of these two pathways. Radiorespirometric and enzymatic analyses clearly demonstrate that the Embden-Meyerhof-Parnas pathway does not function. Studies on the oxidation of pyruvic, acetic, succinic, and glutamic acids show that terminal respiration includes both the tricarboxylic acid and glyoxylic acid cycles.