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Induction of embryogenesis in isolated microspores of artichoke (Cynara scolymus L.)

Stamigna, C., Crinò, P., Chatelet, P., Saccardo, F.
Acta horticulturae 2004 no.660 pp. 139-143
Brassica, Cynara cardunculus, antioxidants, artichokes, ascorbic acid, cell division, chlorogenic acid, citric acid, clones, cysteine, embryogenesis, genotype, in vitro culture, microspores
Our work aimed at improving in vitro culture of artichoke microspores and at obtaining their further embryogenic development by appropriately modifying the protocol already applied on Brassica. Microspores both at the late unicellular and at the early bicellular stages were homogenised in B5 wash substrate containing different antioxidants (cysteine, chlorogenic acid, ascorbic acid, and citric acid) and then cultured on NLND medium modified for different organic compounds. The induction phase (48 h at 30 and 18°) was followed by in vitro dark culture at 18, 25 and 30°. As for other species, the artichoke genotype seems to play an important role either in the microspore culture or in their embryogenic response. The highest percentage of induction was observed in late unicellular microspores isolated both from a French and an Italian genotype, when using cysteine in the extraction medium. The first cell division and the few subsequent ones were noticed only for one of these clones. Although the not yet optimised in vitro culture conditions could have influenced negatively cell development, the results obtained up to now seem promising for future activities.