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Diagnosis of artichoke latent virus (ARLV) and other relevant viruses in "late" artichoke germoplasm

Pasquini, G., Lumia, V., Barba, M.
Acta horticulturae 2004 no.660 pp. 497-500
germplasm, nucleic acid hybridization, diagnostic techniques, Artichoke latent virus, artichokes, reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, viruses, Artichoke mottled crinkle virus, Artichoke Italian latent virus, commercialization, Cucumber mosaic virus, Tomato spotted wilt orthotospovirus, pathogens, viroids
In the last years new international rules have been issued with the aim to set up the commercialisation and sanitary requirements necessary for the safe movement of germplasm around the world. Of course the most dangerous pathogens, essentially the systemic ones (viruses, viroids, phytoplasmas) must be excluded from material being transported. In this paper our experience is reported in establishing the most reliable and sensitive serological and molecular protocols to exclude the presence of the most important viruses causing severe problems in the cultivation of artichoke. Main attention has been focused in detecting ArLV, the most spread virus, whose control is compulsory for obtaining sanitary selected material. Different diagnostic methods (one-step RT-PCR, tissue imprint molecular hybridization with DNA probe) have been applied in detecting ArLV both in vivo and in vitro cultured 'late' artichoke germplasm. Contemporarily, RT-PCR procedures and ELISA tests have been evaluated on a large number of field samples for the diagnosis of Artichoke mottled crinkle virus (AMCV), Artichoke Italian latent virus (AILV), Tomato spotted wilt virus (TSWV), Inpatiens necrotic spot virus (INSV) and Cucumber mosaic virus (CMV).