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Effects of Pectin Pentaoligosaccharide from Hawthorn (Crataegus pinnatifida Bunge. var. Major) on the Activity and mRNA Levels of Enzymes Involved in Fatty Acid Oxidation in the Liver of Mice Fed a High-Fat Diet

Author:
Li, Tuo-ping, Zhu, Ru-gang, Dong, Yin-ping, Liu, Yong-hui, Li, Su-hong, Chen, Gang
Source:
Journal of agricultural and food chemistry 2013 v.61 no.31 pp. 7599-7605
ISSN:
1520-5118
Subject:
Crataegus pinnatifida, acyl coenzyme A, cardiovascular diseases, carnitine palmitoyltransferase, diet therapy, enzyme activity, excretion, fatty acids, feces, gene expression regulation, genes, high fat diet, ingestion, liver, messenger RNA, mice, obesity, oxidation, pectins, protein synthesis, weight gain
Abstract:
The regulatory effects of haw pectin pentaoligosaccharide (HPPS) on fatty acid oxidation-related enzyme activities and mRNA levels were investigated in the liver of high fat diet induced hyperlipidemic mice. Results showed that HPPS (150 mg/kg for 10 weeks) significantly suppresses weight gain (32.3 ± 0.26 and 21.1 ± 0.14 g for high-fat diet and HPPS groups, respectively), decreases serum triacylglycerol levels (1.64 ± 0.09 and 0.91 ± 0.02 mmol/L, respectively), and increases lipid excretion in feces (55.7 ± 0.38 and 106.4 ± 0.57 mg/g for total lipid, respectively), compared to high-fat diet as control. HPPS significantly increased the hepatic fatty acid oxidation-related enzyme activities of acyl-CoA oxidase, carnitine palmitoyltransferase I, 3-ketoacyl-CoA thiolase, and 2,4-dienoyl-CoA reductase by 53.8, 74.2, 47.1, and 24.2%, respectively. Meanwhile, the corresponding mRNAs were up-regulated by 89.6, 85.8, 82.9, and 30.9%, respectively. Moreover, HPPS was able to up-regulate the gene and protein expressions of peroxisome proliferator-activated receptor α. Results suggest that continuous HPPS ingestion may be used as dietary therapy to prevent obesity and cardiovascular diseases.
Agid:
322209