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Development of an Indirect ELISA to Detect Humoral Response to Flavobacterium columnare Infection of Channel Catfish, Ictalurus punctatus
- Shoemaker, Craig A., Shelby, Richard A., Klesius, Phillip H.
- Journal of applied aquaculture 2003 v.14 no.1-2 pp. 43-52
- Flavobacterium columnare, Ictalurus punctatus, agglutination, antibodies, antigens, blood serum, catfish, enzyme-linked immunosorbent assay, goats, humoral immunity, immunoglobulin G, immunoglobulin M, monitoring, rabbits, vaccination
- The humoral antibody response of channel catfish, Ictalurus punctatus, to experimental Flavobacterium columnare infection was measured in control (non-infected) and infected (intraperitoneal- and intramuscular-injected) fish by an indirect enzyme-linked immunoassay (ELISA). The antibody response of the experimentally infected fish was significantly higher (P<0.0001) than that of the non-infected channel catfish by both indirect ELISA and agglutination. The indirect ELISA utilized goat anti-channel catfish IgM and a commercially available rabbit anti-goat IgG enzyme conjugate. The antibody response was directed against a cell-free sonicated antigen preparation derived from live whole F. columnare cells. Indirect ELISA and agglutination results correlated (r2 = 0.60) for anti-F. columnare antibody response in experimentally and non-infected fish. We were also able to correlate ELISA and agglutination results of 60 fish naturally infected with F. columnare(r2 = 0.76). Indirect ELISA will allow for rapid monitoring of humoral antibody, following natural exposure or vaccination against F. columnare, with a small sample of serum.