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Two-Stage Statistical Medium Optimization for Augmented Cellulase Production via Solid-State Fermentation by Newly Isolated Aspergillus niger HN-1 and Application of Crude Cellulase Consortium in Hydrolysis of Rice Straw
- Sandhu, Simranjeet
Kaur, Oberoi, Harinder Singh, Babbar, Neha, Miglani, Kanupriya, Chadha, Bhupinder Singh, Nanda, Dhiraj Kumar
- Journal of agricultural and food chemistry 2013 v.61 no.51 pp. 12653-12661
- Aspergillus niger, alpha-N-arabinofuranosidase, arabinose, beta-glucosidase, biomass, calcium chloride, cellulose 1,4-beta-cellobiosidase, computer software, endo-1,4-beta-glucanase, glucose, hydrolysis, potassium dihydrogen phosphate, rice straw, solid state fermentation, trace elements, urea, xylan, xylan 1,4-beta-xylosidase, xylose
- Cellulolytic enzyme production by newly isolated Aspergillus niger HN-1 was statistically optimized using Plackett–Burman and central composite design (CCD). Optimum concentrations of 2, 0.40, 0.01, and 0.60 g L –¹ for KH₂PO₄, urea, trace elements solution, and CaCl₂·2H₂O, respectively, were suggested by Design-Expert software. The two-stage optimization process led to a 3- and 2-fold increases in the filter paper cellulase (FP) and β-glucosidase activities, respectively. FP, β-glucosidase, endoglucanase, exopolygalaturonase, cellobiohydrolase, xylanase, α-l-arabinofuranosidase, β-xylosidase, and xylan esterase activities of 36.7 ± 1.54 FPU gds–¹, 252.3 ± 7.4 IU gds–¹, 416.3 ± 22.8 IU gds–¹, 111.2 ± 5.4 IU gds–¹, 8.9 ± 0.50 IU gds–¹, 2593.5 ± 78.9 IU gds–¹, 79.4 ± 4.3 IU gds–¹, 180.8 ± 9.3 IU gds–¹, and 288.7 ± 11.8 IU gds–¹, respectively, were obtained through solid-state fermentation during the validation studies. Hydrolysis of alkali-treated rice straw with crude cellulases resulted in about 84% glucan to glucose, 89% xylan to xylose, and 91% arabinan to arabinose conversions, indicating potential for biomass hydrolysis by the crude cellulase consortium obtained in this study.