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Two-Stage Statistical Medium Optimization for Augmented Cellulase Production via Solid-State Fermentation by Newly Isolated Aspergillus niger HN-1 and Application of Crude Cellulase Consortium in Hydrolysis of Rice Straw

Sandhu, Simranjeet Kaur, Oberoi, Harinder Singh, Babbar, Neha, Miglani, Kanupriya, Chadha, Bhupinder Singh, Nanda, Dhiraj Kumar
Journal of agricultural and food chemistry 2013 v.61 no.51 pp. 12653-12661
Aspergillus niger, alpha-N-arabinofuranosidase, arabinose, beta-glucosidase, biomass, calcium chloride, cellulose 1,4-beta-cellobiosidase, computer software, endo-1,4-beta-glucanase, glucose, hydrolysis, potassium dihydrogen phosphate, rice straw, solid state fermentation, trace elements, urea, xylan, xylan 1,4-beta-xylosidase, xylose
Cellulolytic enzyme production by newly isolated Aspergillus niger HN-1 was statistically optimized using Plackett–Burman and central composite design (CCD). Optimum concentrations of 2, 0.40, 0.01, and 0.60 g L –¹ for KH₂PO₄, urea, trace elements solution, and CaCl₂·2H₂O, respectively, were suggested by Design-Expert software. The two-stage optimization process led to a 3- and 2-fold increases in the filter paper cellulase (FP) and β-glucosidase activities, respectively. FP, β-glucosidase, endoglucanase, exopolygalaturonase, cellobiohydrolase, xylanase, α-l-arabinofuranosidase, β-xylosidase, and xylan esterase activities of 36.7 ± 1.54 FPU gds–¹, 252.3 ± 7.4 IU gds–¹, 416.3 ± 22.8 IU gds–¹, 111.2 ± 5.4 IU gds–¹, 8.9 ± 0.50 IU gds–¹, 2593.5 ± 78.9 IU gds–¹, 79.4 ± 4.3 IU gds–¹, 180.8 ± 9.3 IU gds–¹, and 288.7 ± 11.8 IU gds–¹, respectively, were obtained through solid-state fermentation during the validation studies. Hydrolysis of alkali-treated rice straw with crude cellulases resulted in about 84% glucan to glucose, 89% xylan to xylose, and 91% arabinan to arabinose conversions, indicating potential for biomass hydrolysis by the crude cellulase consortium obtained in this study.