Jump to Main Content
Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat
- Kemp, Gabré, Bergmann, Carl W., Clay, Ron, Westhuizen, Amie J. van der, Pretorius, Zacharia A.
- Molecular plant-microbe interactions 2003 v.16 no.11 pp. 955-961
- Bipolaris sorokiniana, Triticum aestivum, amino acid sequences, cell walls, fungi, leaves, pathogens, polygalacturonase, stems, wheat
- Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.