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Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum

Author:
GIRAUD, T., YOCKTENG, R., MARTHEY, S., CHIAPELLO, H., JONOT, O., LOPEZ-VILLAVICENCIO, M., DE VIENNE, D.M., HOOD, M.E., REFREGIER, G., GENDRAULT-JACQUEMARD, A., WINCKER, P., DOSSAT, C.
Source:
Molecular ecology notes 2008 v.8 no.2 pp. 387-392
ISSN:
1471-8278
Subject:
DNA libraries, Dianthus carthusianorum, Microbotryum, Silene latifolia, expressed sequence tags, fungi, genetic markers, microsatellite repeats, population genetics, sibling species
Abstract:
We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M. dianthorum, collected, respectively, from the plants Silene latifolia, S. nutans, S. vulgaris and Dianthus carthusianorum. Intrapopulation polymorphism was investigated using 24 isolates, and cross-amplification was explored using 23 isolates belonging to at least 10 different Microbotryum species. This study provides numerous microsatellite markers for population genetics and mapping studies.
Agid:
3397115