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Development of multiple dominant markers by using Vectorette PCR-based nonradioactive transposable element display
- WANG, JIANJUN, MILLER, THOMAS A., PARK, YOONSEONG
- Molecular ecology notes 2006 v.6 no.3 pp. 642-645
- Heliothis virescens, gels, insects, polymerase chain reaction, radiolabeling, transposons
- Transposable element (TE) display is a method exhibiting the insertion site variations of TE, serving for multiple dominant markers. We report an improved TE display method evaluated by testing inheritance pattern of the TE insertion sites in two different piggyBac-like elements in a lepidopteran insect tobacco budworm. The specificity of the polymerase chain reaction (PCR)-based method was confirmed by sequencing randomly chosen a total of 15 bands all revealing the junctions between TE-end and the flanking sequence. The improved method removes the steps in the conventional TE display method using radiolabelled primers and running denaturing gel, saving time and costs.