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Newly developed polymorphic microsatellite markers in Job's tears (Coix lacryma-jobi L.)

Author:
MA, KYUNG-HO, KIM, KWANG-HO, DIXIT, ANUPAM, YU, JAE-WOONG, CHUNG, JONG-WOOK, LEE, JEONG-HEUI, CHO, EUN-GI, KIM, TAE SAN, PARK, YONG-JIN
Source:
Molecular ecology notes 2006 v.6 no.3 pp. 689-691
ISSN:
1471-8278
Subject:
Coix lacryma-jobi, alleles, gene banks, genetic markers, genetic variation, germplasm, heterozygosity, loci, microsatellite repeats, screening
Abstract:
A microsatellite-enriched library of Job's tears (Coix lacryma-jobi L. var. Ma-yuen Stapf) was constructed using a modified biotin-streptavidin capture method. After screening, 17 polymorphic microsatellites were used for diversity analysis among 30 Job's tears accessions. The number of alleles ranged from one to five alleles per locus with an average of 2.8 alleles. Expected heterozygosity (HE) and polymorphism information content (PIC) ranged from 0 to 0.676 and from 0 to 0.666, respectively. The newly developed microsatellite markers are expected to be very valuable tools for evaluation of genetic diversity among large germplasm collection of Job's tears present in our Korean Gene Bank.
Agid:
3397767