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Characterization of 19 single nucleotide polymorphism markers for coho salmon
- SMITH, C.T., PARK, L., VANDOORNIK, D., SEEB, L.W., SEEB, J.E.
- Molecular ecology notes 2006 v.6 no.3 pp. 715-720
- Oncorhynchus kisutch, gene frequency, genotyping, loci, mitochondrial DNA, nuclear genome, nucleotide sequences, single nucleotide polymorphism
- We report 39 single nucleotide polymorphisms (SNPs) observed in 23 nuclear DNA sequences in coho salmon Oncorhynchus kisutch. High-throughput genotyping assays based on the 5'-nuclease reaction were developed for 17 of these nuclear SNPs and for two previously published mitochondrial DNA SNPs. Minor allele frequency differences (Δq) among collections were between 5.2% and 51.2%, resulting in per locus FST estimates of 0.00-0.24 with an average of 0.09.