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Identification of polymorphic microsatellite markers from RAPD product in turbot (Scophthalmus maximus) and a test of cross-species amplification
- LIU, Y.G., LIU, L.X., LEI, Z.W., GAO, A.Y., LI, B.F.
- Molecular ecology notes 2006 v.6 no.3 pp. 867-869
- DNA, Scophthalmus maximus, alleles, genetic markers, loci, microsatellite repeats, random amplified polymorphic DNA technique, turbot
- Five polymorphic microsatellite loci have been isolated and characterized from random amplified polymorphic DNA product in turbot, Scophthalmus maximus. Twelve microsatellites were selected for designing microsatellite primers, of which five gave working primer pairs. They had between four and nine alleles. Observed and expected heterozygosities varied from 0.76 to 0.90 and from 0.63 to 0.83, respectively. Five additional fish species assessed for cross-species amplification revealed between one and three positive amplifications and between zero and two polymorphic loci per species.