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Limited cross-species microsatellite amplification and the isolation and characterization of new microsatellite markers for the greater stick-nest rat (Leporillus conditor)

Author:
BARCLAY, S.D., COSTELLO, B., SHERWIN, W.B.
Source:
Molecular ecology notes 2006 v.6 no.3 pp. 882-885
ISSN:
1471-8278
Subject:
Mus musculus, Rattus rattus, alleles, genetic markers, genomic libraries, loci, microsatellite repeats, rats
Abstract:
The greater stick-nest rat (Leporillus conditor) is a conilurid rodent whose recent history provides an opportunity to examine genetic changes in reintroduced populations. We trialled 63 known microsatellite primers from Rattus rattus and Mus musculus in L. conditor. Three primer pairs produced polymorphic loci (number of alleles = 2-3, mean, HE = 0.42). Subsequently, we isolated and characterized 12 novel polymorphic microsatellites (mean number of alleles = 5-16, mean HE = 0.76) from L. conditor from genomic libraries for use in population genetic studies.
Agid:
3397825