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Isolation and characterization of microsatellite loci from the rust pathogen, Melampsora lini
- BARRETT, LUKE G., BRUBAKER, CURT L.
- Molecular ecology notes 2006 v.6 no.3 pp. 930-932
- DNA, Linum usitatissimum, Melampsora lini, alleles, clones, loci, microsatellite repeats, pathogens
- We developed and characterized primers for 11 variable microsatellite loci present in the genome of the flax rust, Melampsora lini. The microsatellite loci were identified by sequencing clones from a library of EcoRI DNA fragments enriched for four simple sequence repeat motifs (AAG, AAT, TC and TG). All 11 primer pairs successfully amplified DNA fragments from a sample of 102 M. lini isolates (98 isolated from Linum marginale and four from Linum usitatissimum), revealing a total of 32 alleles. Allelic diversity at the 11 loci ranged from 0.030 to 0.449.