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Multi-residue determination of antibiotics in fish by liquid chromatography-tandem mass spectrometry
- Gbylik, M., Posyniak, A., Mitrowska, K., Bladek, T., Zmudzki, J.
- Food additives & contaminants 2013 v.30 no.6 pp. 940-948
- acetonitrile, aminoglycosides, beta-lactams, fish, fluoroquinolones, macrolides, mass spectrometry, multiresidue analysis, sulfonamides, tetracyclines, trimethoprim
- A multi-residue method for the determination of 34 antibacterial drugs (three aminoglycosides, nine β-lactams, nine fluoroquinolones, three macrolides, five sulfonamides, trimethoprim and four tetracyclines) in fish samples by LC-MS/MS was developed. The procedure enables the isolation of residues based on double extraction of the fish sample with m-phosphoric acid and heptafluorobutyric acid as an ion-pair agent and acetonitrile. All compounds were determined at the same time on a C18 column with gradient elution. The method was validated according to the requirements of European Decision 2002/657/EC. Specificity, recovery, repeatability, within-laboratory reproducibility, decision limit CCα and detection capability CCβ were calculated. CCα ranged from 55.3 to 1085 µg kg⁻¹ and CCβ ranged from 59.5 to 1141 µg kg⁻¹. The overall recoveries were from 96% to 111% with respect to the internal standards.