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Reciprocal compatibility within the genus Pisum L. as studied in F1 hybrids: 1. Crosses involving P. sativum L. subsp. sativum

Author:
Kosterin, O. E., Bogdanova, V. S.
Source:
Genetic resources and crop evolution 2015 v.62 no.5 pp. 691-709
ISSN:
0925-9864
Subject:
Pisum fulvum, Pisum sativum subsp. abyssinicum, Pisum sativum subsp. elatius, Pisum sativum subsp. sativum, biomass, crossing, embryogenesis, hybrids, parents, peas, plastid DNA, pollen, seeds, taxonomy, vigor
Abstract:
Seven diverse accessions of peas including Pisum fulvum Boiss. et Noë (WL2140), Pisum abyssinicum A. Br. (VIR2759) and Pisum sativum L. subsp. elatius (Bieb.) Schmahl. were crossed reciprocally with a testerline WL1238 of the cultivated pea, P. sativum L. subsp. sativum. Efficiency of crosses (the average number of hybrid seeds per cross) in reciprocal directions and general characteristics of reciprocal F1 hybrids, such as F1 seed mass, pollen and seed fertility, height, yield and biomass, were compared. P. fulvum and P. abyssinicum showed strong reproductive barriers with P. sativum subsp. sativum, both prezygotic, as estimated by crossing efficiency, and postzygotic, manifested as hybrid sterility and weakness. Among crosses of five accessions of wild P. sativum subsp. elatius with the testerline, only hybrids with CE1 (Crimea) showed no difference in reciprocal combinations. Reciprocal hybrids with CE1 and JI1794 (Holan Heights) showed fully fertile pollen. In other cases reciprocal differences were registered as to pollen fertility and general vigour. Two special cases with wild peas as seed parents were observed: abnormal hybrids with VIR320 (Palestine) and arrest of embryo development in crosses with L100 (Be’er Sheva). In all cases of reciprocal differences except for those involving accession 721 (Mt. Carmel), vigour and fertility of hybrids were higher when WL1238 (P. sativum subsp. sativum) was used as seed parent. Paternal plastid DNA was registered in hybrids with WL2140 and VIR320 as seed parents. Reproductive barriers within P. sativum L. showed a complicated pattern scarcely corresponding to any intraspecies taxonomy.
Agid:
3406667